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The Description Of HPLC
Risun Bio-Tech Inc | Updated: Sep 21, 2016

the description of HPLC


   HPLC has become a backbone of synthetic and analytical chemistry, meaning any serious lab has at least one HPLC system. 

Due to the broad range of different compounds and solvents everyday chemists have to work with it is becoming increasingly 

important that HPLC systems are versatile enough to meet researchers needs.


   KNAUER are experts in HPLC and provide modular HPLC systems which can be easily integrated into existing analytical 

equipment and adjusted depending on the seperation required.


   AZoM spoke to, John Rohloff, product manager for analytical HPLC at KNAUER, about the HPLC systems that KNAUER 

provide and the expert advice they offer researchers on achieving the best seperation possible.


   HPLC is a major separation technique. Could you please introduce our readers to HPLC?


   HPLC, High Performance Liquid Chromatography, is one of the standard analysis techniques used in nearly every laboratory.


   As the name implies, it uses a liquid solvent as an eluent. Analytes are separated as they pass through the chromatographic column 

according to their chemical and physical properties and characterized after seperation using different detection principles.


   A stationary phase, which typically consists of a chemically modified silica gel, induces the separation of the analytes. Afterwards, 

the analytes separately enter a detection unit, which is usually a UV/VIS-detector, which measures the absorption of light of each 

analyte at a specified wavelength.


   Recently UV detectors have been being replaced by mass spectrometers which allow highly accurate identifications.